What is MIQE?
The technical standard of most publications utilising qPCR is either difficult to judge, since insufficient information is provided in the "Materials and Methods" sections of most papers, or is poor, since normalisation procedures are inappropriate, most commonly using a single, unvalidated reference gene. The implications of this are disconcerting, since the peer-reviewed scientific literature forms the bedrock of current knowledge and provides the starting point for future experiments.
The MIQE guidelines aim to
The guidelines have been cited 1546 times in the peer-reviewed literature, as of mid-June 2013. It is the fifth most cited paper in Clinical Chemistry, and the only one of these published this century.
The MIQE guidelines aim to
- enable authors to design and report qPCR experiments that have greater inherent value
- allow reviewers and editors to measure the technical quality of submitted manuscripts against an established yardstick.
- facilitate easier replication of experiments de- scribed in published studies that follow these guidelines.
The guidelines have been cited 1546 times in the peer-reviewed literature, as of mid-June 2013. It is the fifth most cited paper in Clinical Chemistry, and the only one of these published this century.
MIQE for digital PCR
dMIQE aims to
The rapid and universal adoption of the dMIQE guidelines should result in more reproducible data and reliable scientific reporting that will increase the im- pact of the associated research and maximize the con- tributions of this promising and rapidly developing technology.
- enable authors to design, perform, and report dPCR experiments that have greater scientific integrity
- facilitate replication of experiments that are de- scribed in published studies in which these guidelines were followed
- provide critical information that allows review- ers and editors to measure the technical quality of submitted manuscripts against an established standard.
The rapid and universal adoption of the dMIQE guidelines should result in more reproducible data and reliable scientific reporting that will increase the im- pact of the associated research and maximize the con- tributions of this promising and rapidly developing technology.