Welcome to the MIQE website.
The technical standard of most publications utilising qPCR to quantify gene expression is either difficult to judge, since very little information is provided in the "Materials and Methods" sections of most papers, or is poor, since normalisation procedures are inappropriate, most commonly using a single, unvalidated reference gene. The implications of this are disconcerting, since the peer-reviewed scientific literature forms the bedrock of current knowledge and provides the starting point for future experiments.
This site aims to
- assemble information relevant to qPCR
- review reagents, plastic ware, instruments, meetings and literature
Stephen Bustin
|
